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本文介绍快速测定庆大霉素的含量是在碱性介质中庆大霉素与二价铜离子形成有色复合物,从而减少单一成分分析所需的时间.碱性酒石酸铜(ACT)试液的制备:每毫升含100μmol硫酸铜,210μmol酒石酸钾钠和1.25mmol氢氧化钠.测定方法:将安瓿中硫酸庆大霉素注射液(40mg/ml)移至一清洁干燥容器中,精密吸取0.3ml于试管内,加水0.7ml和ACT试液1.0ml,混匀;同时作空白对照,在560nm波长处测定吸收度.样品测定结
This article describes the rapid determination of gentamicin content is the formation of a colored complex of gentamicin and divalent copper ions in alkaline medium, thereby reducing the time required for the single component analysis of alkaline copper tartrate (ACT) test solution Preparation: Containing 100 μmol of copper sulfate, 210 μmol of potassium sodium tartrate and 1.25 mmol of sodium hydroxide per ml Method of determination: Gentamicin sulfate injection (40 mg / ml) in ampoule was transferred to a clean and dry container and precision pipetted 0.3 ml In the test tube, add 0.7ml of water and 1.0ml of ACT test solution, and mix well; at the same time, make a blank control and measure the absorbance at the wavelength of 560nm.