用5、10、15、20和25天龄大鼠睾丸曲细精管分离细胞制成悬液,在体外经固体琼脂培养和旋转培养,得到圆球状和索状细胞团聚体,其主要由支持细胞组成,管周细胞包绕在支持细胞外周。10天龄以前组,在团聚体中可见到部分生精细胞,15天龄以后组的生精细胞全部位于团聚体外.表明新生大鼠睾丸支持细胞、管周细胞及生精细胞间仍保留着部分胚胎期的识别和选择性粘附能力。 Cells were isolated from testicular seminiferous tubules of 5, 10, 15, 20, and 25 days old testes and cultured in solid agar and in vitro to obtain spherical and cord-like cell aggregates, Cell composition, peritubular cells surround the periphery of the supporting cells. Groups of spermatogenic cells were seen in the aggregates 10 days before age, and all the spermatogenic cells in the group after 15 days old were located outside the agglomerates, indicating that the testicular cells, the pericytes and the spermatogenic cells of neonatal rats remained Partial embryonic recognition and selective adhesion.