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目的：癌基因结构及表达异常与肿瘤的发生发展有着密切的关系，作者在于考察胶质瘤中ｃ－ｍｙｃ基因的状况．方法：采用Ｓｏｕｔｈｅｒｎｂｌｏｔ杂交和ＲＮＡ打点杂交的方法以正常人脑组织为对照观察了７例人脑胶质瘤组织中ｃ－ｍｙｃ基因在ＤＮＡ和ＲＮＡ水平的改变．结果：７例人脑胶质瘤标本中ｃ－ｍｙｃ基因有不同程度的扩增和重排，其ｃ－ｍｙｃｍＲＮＡ表达幅度是正常组织的１．６～４．０倍，结论：在胶质瘤中有ｃ－ｍｙｃ基因的异常．
OBJECTIVE: The oncogene structure and abnormal expression are closely related to the occurrence and development of tumors. The purpose of this study is to investigate the status of c-myc gene in gliomas. Methods: Southern blot and RNA dot blot were used to detect the changes of c-myc mRNA and RNA in seven human glioma tissues. Results: The c-myc gene was amplified and rearranged in 7 human glioma specimens in varying degrees and the expression of c-myc mRNA was 1.6-4.0 times of the normal tissue. Conclusion: In gliomas In c-myc gene abnormalities.