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目的：建立测定杭栓保心片中丹参酮ⅡＡ含量的方法。方法：采用反相高效液相色谱法，以甲醇作溶剂，水浴加热回流提取。ＬＵＮＡＣ１８色谱柱分离，流动相为甲醇水（８５∶１５），流速０．８ｍｌ／ｍｉｎ，检测波长２７０ｎｍ。结果：丹参酮ⅡＡ与其相邻峰的分离度是２．０；理论板数按丹ⅡＡ峰计算是４３８８２；丹参酮ⅡＡ对照品线性范围２．８３０～４５．２８μｇ／ｍｌ，ｒ＝０．９９９９；平均回收率９９．５％，ＲＳＤ＝２．５％（ｎ＝５）。结论：用本法测定抗栓保心片中丹参酮ⅡＡ含量，操作简便，结果准确
Objective: To establish a method for the determination of tanshinone IIA in Hangzhou Huaxin Baoxin Tablets. Methods: Reversed-phase high performance liquid chromatography was used with methanol as the solvent and reflux extraction with water bath. The LUNAC18 column was separated, the mobile phase was methanol-water (85:15), the flow rate was 0.8 ml/min, and the detection wavelength was 270 nm. RESULTS: The resolution of tanshinone IIA and its adjacent peaks was 2.0; the number of theoretical plates was 43882 calculated from Dan IIA peak; the linear range of tanshinone IIA control was 2.830 to 45.28 μg/ml, r=0.9999; The recovery was 99.5% with RSD=2.5% (n=5). Conclusion: This method is used to determine the content of tanshinone IIA in Kangshuanbaoxin Tablet. The operation is simple and the result is accurate.