以黄酮得率为考察指标,通过单因素试验对可能影响黄酮分离纯化的因素进行研究分析,确定了纯化沙棘籽及果皮渣黄酮的最佳大孔树脂种类及其最佳工艺条件。纯化的最佳树脂为D101大孔树脂,其最佳工艺条件:上样液浓度6mg/mL、洗脱浓度60%乙醇、乙醇洗脱速率3mL/min和洗脱剂用量为300mL。经树脂分离纯化后的沙棘籽及果皮渣黄酮洗脱率为91.09%,纯度为76.4%。 Taking the yield of flavonoids as index, the factors which may affect the separation and purification of flavonoids were studied by single factor experiment, and the best macroporous resin types and the optimum technological conditions were determined. The optimum resin was D101 macroporous resin. The optimum conditions were as follows: the concentration of sample solution was 6mg / mL, the concentration of ethanol was 60%, the elution rate of ethanol was 3mL / min and the amount of eluent was 300mL. Separation and purification of resin Seabuckthorn seeds and peel residue flavonoids elution rate of 91.09%, a purity of 76.4%.