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目的 以四氯化碳 (CCl4 )染毒大鼠为肝损伤动物模型 ,观察了胡萝卜、西红柿、甘蓝、莴苣、芹菜、洋葱、茄子、菠菜等 8种蔬菜的混合菜汁对大鼠脂质过氧化和转氨酶活性的影响 ,并探讨其可能机制。方法 将 110只大鼠随机分为 5组 :对照组、CCl4 组、CCl4 +提前给予混合蔬菜汁组、CCl4 + 10 0 %混合蔬菜汁组、CCl4 + 5 0 %混合蔬菜汁组。CCl4 按0 3 5ml kg 1次性腹腔注射 ,混合蔬菜汁按 1ml 10 0g体重灌胃。分别于染毒后第 1天、第 2天、第 7天将各组大鼠随机处死 6只 ,测定血清丙氨酸转氨酶 (ALT)、天冬氨酸转氨酶 (AST)活性 ,血清脂质过氧化 (LPO)水平 ,血清铜蓝蛋白 (CP)含量 ,血清、肝匀浆超氧化物歧化酶 (SOD)活性 ,血清、肝匀浆过氧化氢酶 (CAT)活性。结果 各染毒组肝 体比值增加 ,血清ALT、AST活性增高 ,LPO水平升高 ,各混合蔬菜汁组血清AST、ALT活性均低于CCl4 组 ,以 5 0 %组血清AST、ALT活性最低。结论 在染毒同时给予 5 0 %混合蔬菜汁 ,对CCl4 引起LPO有明显的拮抗作用 ,可能与其能清除·CCl3有关。
OBJECTIVE To investigate the effects of mixed vegetable juice of 8 vegetables including carrot, tomato, cabbage, lettuce, celery, onion, eggplant and spinach on liver injury in rats exposed to carbon tetrachloride (CCl4) Oxidative and transaminase activity, and explore its possible mechanism. Methods 110 rats were randomly divided into 5 groups: control group, CCl4 group, CCl4 + group, mixed vegetable juice group, CCl4 + 100% mixed vegetable juice group and CCl4 + 50% mixed vegetable juice group. CCl4 by 0 3 5ml kg 1 intraperitoneal injection, mixed vegetable juice by 1ml 10g body weight gavage. Six rats were randomly sacrificed on the 1st day, the 2nd day and the 7th day after exposure. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) activity, serum lipid (LPO), serum ceruloplasmin (CP), serum and liver homogenate superoxide dismutase (SOD) activity, serum and liver homogenate catalase (CAT) activity. Results The ratio of liver and body increased with the increase of serum ALT and AST levels and LPO level. The serum AST and ALT activities in each mixed vegetable juice group were lower than those in CCl4 group. Serum AST and ALT activities were lowest in 50% of the mixed vegetable juice groups. Conclusion The application of 50% mixed vegetable juice at the same time of exposure could obviously antagonize LPO induced by CCl4, which may be related to its ability to eliminate · CCl3.