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目的 :清热中药大多具有一定的解热作用 ,为探讨其解热作用机理 ,我们选用典型的清热中药黄芩、银花、连翘组成清热方 ,从体温调节中枢神经介质方面来研究其解热作用机制。方法 :选用Wistar大鼠 ,按体重随机分作 3组 ,背部皮下注射酵母混悬液制造发热模型 ,给药组灌服清热方颗粒剂 ,模型组予等量饮用水 ,正常对照组不作处理。给药 2h后断头取脑 ,用放免方法检测大鼠下丘脑组织中AVP含量。结果 :模型组大鼠体温和下丘脑组织中AVP含量均高于正常对照组 ,给药组大鼠体温低于模型组 ,而给药组大鼠下丘脑组织中AVP含量高于模型组。结论 :实验结果表明AVP参与了酵母致大鼠发热的发热反应 ,影响体温调节中枢解热介质AVP代谢 ,增加其含量 ,从而抑制体温调节点上移是清热中药解热作用机理之一。
Objective: Heat-clearing traditional Chinese medicines mostly have a certain antipyretic effect. In order to explore the mechanism of antipyretic action, we use the traditional heat-clearing herbs Astragalus membranaceus, Yinhua, and Forsythia to form a heat-removal method, and study the antipyretic effects of body temperature regulation on central nervous system media. mechanism. Methods: Wistar rats were randomly divided into 3 groups according to body weight. Subcutaneous subcutaneous injection of yeast suspension was used to produce a fever model. The administration group was fed with Qingrefang granules and the model group was given equal drinking water. The normal control group was not treated. After 2 hours of drug administration, the brain was removed and the content of AVP in the hypothalamus was detected by radioimmunoassay. RESULTS: The body temperature and hypothalamic tissue AVP content in the model group were higher than those in the normal control group. The body temperature in the administration group was lower than that in the model group, but the AVP content in the hypothalamus tissue in the administration group was higher than that in the model group. Conclusion : The experimental results show that AVP is involved in the fever reaction of yeast-induced fever in rats, which affects the metabolism of AVP in the body’s central nervous system and increases the content of AVP. Therefore, inhibiting the upward shift of body temperature regulation point is one of the mechanisms of antipyretic effect of heat-clearing traditional Chinese medicine.