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为进一步探讨子宫颈癌的分子发病机制，采用PCR、ISH和IHC方法对51例子宫颈癌组织的HPV16感染、HPV16E6mRNA、p53、p21ras和c－myc蛋白表达分布进行了研究。结果表明51例中有HPV16感染者38例，HPV16E6mRNA表达者35例，其阳性细胞主要分布在高分化癌巢之周边部或中、低分化癌之整个癌巢；p53、p21ras和c－myc蛋白阳性检出率分别为33．3％、60．8％和76．5％，其阳性细胞是弥漫或灶状分布。统计分析表明HPV16感染与P53、P21ras、c－myc蛋白积累间无关，但后三者间则显著相关。这提示癌基因活化或抗癌基因的失活及HPV16感染等因素之协同在子宫颈癌的发生发展中起重要作用。
To further explore the molecular pathogenesis of cervical cancer, HPV16 infection, HPV16E6 mRNA, p53, p21ras and c-myc protein expression in 51 cases of cervical cancer tissues were studied by PCR, ISH and IHC methods. The results showed that there were 38 cases of HPV16 infection and 35 cases of HPV16E6 mRNA expression in 51 cases. The positive cells were mainly distributed in the periphery of well-differentiated cancer neoplasms or in the poorly-differentiated cancer neoplasms. The p53, p21ras and c-myc proteins The positive detection rates were 33.3%, 60.8% and 76.5%, respectively. The positive cells were diffuse or focal distribution. Statistical analysis showed that there was no correlation between HPV16 infection and P53, P21ras and c-myc protein accumulation, but the latter three were significantly correlated. This suggests that the activation of oncogenes or inactivation of anti-oncogene and HPV16 infection and other factors play an important role in the occurrence and development of cervical cancer.