铁线莲属含有黄酮成分,用溶剂法萃取不同季节粉绿铁线莲叶、与枝干的总黄酮,以卢丁作标准品,用比色法进行含量测定。结果显示,叶中黄酮含量高,其中8月份的含量最高;枝干中含量少,变化不明显。初步揭示了粉绿铁线莲总黄酮含量随季节变化的趋势。实验采用分光光度法,以芦丁为标准样品测定不同季节不同部位中的总黄酮的含量变化,采用正交实验设计对不同季节粉绿铁线莲不同部位中总黄酮含量进行比较。 Clematis contains flavonoids, Solvent extraction of different seasons powder green clematis leaves, and the total flavonoids stems, with Luding as a standard, with colorimetric assay. The results showed that the content of flavonoids in leaves was high, of which the content was the highest in August; the content of stems was small and the changes were not obvious. Preliminary revealed the trend of the content of total flavonoids of Clematis with season changes. The content of total flavonoids in different parts of different seasons was determined by spectrophotometry and rutin as standard samples. The orthogonal experimental design was used to compare the contents of total flavonoids in different parts of Selaginella versicolor.