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目的:探讨NF-κB信号传导通路中转化生长因子激活激酶1(TAK1)靶点对胃癌AGS细胞系增殖的影响。方法:利用si RNA沉默胃癌AGS细胞系中的TAK1基因,通过Western Blot验证细胞中TAK1基因的沉默情况,双项荧光素酶报告基因系统检测TAK1基因沉默对AGS细胞系NF-κB信号传导通路活性的影响,软琼脂克隆形成实验检测沉默TAK1基因对胃癌AGS细胞系成瘤能力的影响,MTT法检测沉默TAK1基因对胃癌AGS细胞系增殖能力的影响。结果:与对照组Sis-Con细胞相比,TAK1基因沉默组Sir-TAK1-1/Sir-TAK1-2细胞中NF-κB信号传导通路活性明显受抑制;TAK1基因沉默组Sir-TAK1-1/Sir-TAK1-2细胞软琼脂克隆形成的集落数目明显减少,差异具有统计学意义(P<0.05),且细胞生长的速度明显减慢。结论:TAK1是NF-κB信号传导通路激活的关键因子,沉默TAK1基因可以抑制AGS胃癌细胞系的成瘤性和增殖力。
AIM: To investigate the effect of TAK1 target on proliferation of gastric cancer AGS cell line in NF-κB signaling pathway. Methods: TAK1 gene was silenced in gastric cancer AGS cell line by si RNA. The silencing of TAK1 gene was verified by Western Blot. The activity of TAK1 gene silenced by TAK1 gene was detected by double-luciferase reporter gene system in AGS cell line NF-κB signaling pathway The effect of silenced TAK1 gene on the tumorigenicity of AGS cell line was detected by soft agar colony formation assay. The effect of TAK1 gene silencing on the proliferation of gastric cancer AGS cell line was detected by MTT assay. Results: Compared with Sis-Con cells in control group, the activity of NF-κB signaling pathway in Sir-TAK1-1 / Sir-TAK1-2 cells was significantly inhibited in TAK1 silencing group. Sir-TAK1-1 / The number of colonies formed by soft agar colony of Sir-TAK1-2 cells was significantly decreased, the difference was statistically significant (P <0.05), and the cell growth rate was significantly slowed down. Conclusion: TAK1 is a key factor in the activation of NF-κB signaling pathway. Silencing TAK1 gene can inhibit the tumorigenicity and proliferation of AGS gastric cancer cell lines.