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研究了表面活性剂 AOT的浓度及相体积比对 AOT/异辛烷反胶团体系提取脂肪酶的影响。随表面活性剂浓度增加提取率增大 ;表面活性剂浓度一定时 ,p H值低提取率高 ;提取率一定时 ,p H值低所需表面活性剂浓度也低。相体积比 (V油 /V水 )增大 ,提取率增大。初步探讨了提取机理
The effects of surfactant AOT concentration and phase volume ratio on lipase extraction from AOT / iso-octane reverse micelle system were investigated. With the increase of surfactant concentration, the extraction rate increased; when the surfactant concentration is constant, the p H value is low, and the extraction rate is high; and when the extraction rate is constant, the p H value is low, and the surfactant concentration required is also low. Phase volume ratio (V oil / V water) increases, the extraction rate increases. Preliminary study of the extraction mechanism