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目的探究乌头碱与人参皂苷Rg1配伍对正常心肌细胞和心力衰竭心肌细胞活力及细胞内离子的影响。方法原代培养正常心肌细胞,并建立心力衰竭心肌细胞模型,采用MTT比色法筛选乌头碱及人参皂苷Rg1的毒效浓度;采用MTT比色法和试剂盒观察乌头碱和人参皂苷Rg1毒-效及效-效浓度配伍对心肌细胞活力和细胞内Na+,K+,Ca2+,Mg2+浓度的影响。结果人参皂苷Rg1的最小毒性浓度为1×10-5mol/L,药效浓度为1×10-8mol/L;乌头碱药效浓度为1×10-9mol/L;乌头碱与人参皂苷Rg1毒-效浓度配伍可分别减轻乌头碱和人参皂苷Rg1对正常心肌细胞的毒性,且均以1∶2(Ac∶Rg1)配伍效果最佳;两者效-效浓度配伍可增强心力衰竭心肌细胞活力,且以1∶1(Ac∶Rg1)配伍效果最佳。结论乌头碱与人参皂苷Rg1配伍对心肌细胞具有减毒增效作用,其机制与调节心肌细胞内Na+,Mg2+,K+,Ca2+浓度有关。
Objective To investigate the effect of aconitine and ginsenoside Rg1 on the viability and intracellular ion of cardiomyocytes in normal cardiomyocytes and heart failure. Methods Primary cardiomyocytes were cultured and cardiomyocyte models of heart failure were established. MTT colorimetric method was used to screen the toxic effect concentration of aconitine and ginsenoside Rg1. MTT assay and kit were used to observe the effect of aconitine and ginsenoside Rg1 Effects of Toxicity - Efficacy and Efficacy - Effectiveness Concentration on Myocardial Cell Viability and Intracellular Na +, K +, Ca2 + and Mg2 + Concentrations. Results The minimum toxic concentration of ginsenoside Rg1 was 1 × 10-5mol / L, the effective concentration was 1 × 10-8mol / L, the concentration of aconitine was 1 × 10-9mol / L, the concentration of aconitine and ginsenoside Rg1 toxic-effect concentration can reduce the toxicity of aconitine and ginsenoside Rg1 to normal cardiomyocytes, respectively, and the optimal combination of both is 1: 2 (Ac: Rg1). The combination of both effect-effect concentration can enhance heart failure Cardiomyocyte activity, and to 1: 1 (Ac: Rg1) compatibility best. Conclusion The combination of aconitine and ginsenoside Rg1 has an attenuating and synergistic effect on cardiomyocytes, and its mechanism is related to the regulation of Na +, Mg2 +, K + and Ca2 + in cardiomyocytes.