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研究了异硫氰酸异鲁米诺的电致化学发光性质及其反应机理。在ＫＯＨ－ＫＣｌ－Ｈ２Ｏ２（ｐＨ１１．８）介质中，施加三角波脉冲电压使异硫氰酸异鲁米诺产生较强的电致化学发光，其浓度在２．５×１０－１１～１．０ × １０－７ｍｏｌ／Ｌ范围与发光强度成线性关系，检测限为６．２×１０－１２ｍｏｌ／Ｌ．重复１０次测定５．０×１０－９ｍｏｌ／Ｌ异硫氰酸异鲁米诺溶液，相对标准偏差为３．０％。方法用于测定Ａｎｔｉ－ＡＦＰ，取得满意结果。
The electrochemical luminescence properties of isomethandi isothiocyanate and its reaction mechanism were studied. In the KOH-KCl-H2O2 (pH11.8) medium, the application of triangular wave pulse voltage to isothiocyanate isoluminol produced strong electrochemiluminescence, the concentration of 2.5 × 10-11 ~ 1.0 × 10-7mol / L range and luminous intensity of a linear relationship, the detection limit of 6.2 × 10-12mol / L. The determination of 5.0 × 10 -9 mol / L isoluminisothiocyanate solution was repeated 10 times with the relative standard deviation of 3.0%. Method for the determination of Anti-AFP, to obtain satisfactory results.