目的　探讨VEGF反义寡核苷酸作为抗肿瘤血管生成治疗新药的可能性。方法　观察VEGF反义、正义、错义寡核苷酸制备的条件培养基对牛血管内皮细胞生长的抑制作用。应用免疫荧光流式细胞技术观察三者分别对C6胶质瘤细胞VEGF表达的影响。结果　反义寡核苷酸对C6胶质瘤细胞VEGF的表达和内皮细胞生长有明显抑制作用 ,而且抑制作用存在浓度依赖性。结论　反义VEGF寡核苷酸通过抑制C6胶质瘤细胞VEGF的表达 ,进而抑制内皮细胞的生长。 Objective To investigate the possibility of using VEGF antisense oligonucleotide as a novel anti-angiogenic therapeutic agent. Methods The growth of bovine vascular endothelial cells was inhibited by conditioned media prepared with VEGF antisense, sense, and missense oligonucleotides. Immunofluorescence flow cytometry was used to observe the effects of the three on the expression of VEGF in C6 glioma cells, respectively. Results Antisense oligonucleotides significantly inhibited the expression of VEGF and the growth of endothelial cells in C6 glioma cells, and the inhibitory effects were concentration-dependent. Conclusion Antisense VEGF oligonucleotides inhibit the growth of endothelial cells by inhibiting the expression of VEGF in C6 glioma cells.