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为了进一步明确Vip3Aa的作用机制,利用透射电镜观察了棉铃虫4龄幼虫取食含Vip3Aa蛋白饲料后中肠杯状细胞的病理变化,并比较了其病变与取食含Cry1Ac饲料后棉铃虫组织病变的差异。取食含Vip3Aa饲料后,棉铃虫幼虫的中肠杯状细胞逐渐发生病变,主要表现为:微绒毛肿胀、脱落;细胞核核膜界限不清晰,染色质分布不均匀;线粒体变形、数量减少,内脊不清晰;内质网杂乱不规则、数量减少。与取食Cry1Ac的棉铃虫相比,取食Vip3Aa的棉铃虫中肠杯状细胞发生病变较为缓慢,在取食12h后才发现明显病变,随着取食时间的增加病变越来越明显;而取食Cry1Ac的棉铃虫2h后中肠杯状细胞就出现明显病变。本研究可为Vip3Aa作为新毒素策略的重要蛋白在棉铃虫Helicoverpa armigera综合防治中更好地发挥作用提供理论依据。
In order to further clarify the mechanism of action of Vip3Aa, the pathological changes of goblet cells in the midgut of fourth instar larvae of cotton bollworm (Helicoverpa armigera) were observed by transmission electron microscopy. The pathological changes of the midgut goblet cells were compared with those of Cry1Ac fed with Cry1Ac The difference. Mice fed with Vip3Aa gradually developed lesions of midgut goblet cells in the larvae of H. armigera. The main manifestations were: swollen and detached microvilli, unclear boundaries of nuclear and nuclear chromatin, uneven distribution of chromatin, mitochondrial deformation, Ridge is not clear; endoplasmic reticulum disorderly irregular, the number decreases. Compared with the cotton bollworm fed with Cry1Ac, the pathological changes of goblet cells in the midgut of the cotton bollworms fed with Vip3Aa were relatively slow, and no obvious pathological changes were found after 12h of feeding. The lesions became more and more obvious with the increase of feeding time. 2 h after feeding Cry1Ac cotton bollworm midgut goblet cells showed obvious lesions. This study may provide a theoretical basis for Vip3Aa to play a better role in the comprehensive control of Helicoverpa armigera as an important protein of the new toxin strategy.