论文部分内容阅读
目的:建立HPLC-DAD方法,一次性鉴别西红花常见伪品红花、菊花,并测定其掺伪量。方法:采用高效液相色谱法,流动相为乙腈-0.3%磷酸水;波长范围200～410 nm;羟基红花黄色素A检测波长402 nm,绿原酸检测波长327 nm;梯度洗脱。结果:通过HPLC分析,确定羟基红花黄色素A、绿原酸分别为红花、菊花的特征成分,测定样品含量,可鉴别出样品中是否掺入了红花、菊花,并确定伪品掺入量。羟基红花黄色素A 0.005～0.25 mg.mL-1,绿原酸0.001～0.05 mg.mL-1范围内线性关系良好,方法回收率高,重复性好。结论:此方法能一次性鉴别西红花常见伪品红花、菊花,并测定其掺伪量,对其它药材的相关研究也有参考价值。
OBJECTIVE: To establish a HPLC-DAD method to identify saffron and chrysanthemum common to crocus saffron, and to determine the amount of adulteration. Methods: The mobile phase consisted of acetonitrile - 0.3% phosphoric acid water with the wavelength range of 200-410 nm. The detection wavelength of hydroxyl safflower A was 402 nm and the detection wavelength of chlorogenic acid was 327 nm. The gradient elution was performed. Results: By HPLC analysis, the determination of hydroxysafflor yellow A and chlorogenic acid were the characteristic components of safflower and chrysanthemum respectively. The contents of safflower and chrysanthemum were determined. The safflower and chrysanthemum were identified in the sample, Into the amount. Hydroxysafflor yellow A 0.005 ~ 0.25 mg.mL-1, chlorogenic acid 0.001 ~ 0.05 mg.mL-1 within a good linear relationship, the method of high recovery, good repeatability. Conclusion: This method can identify common safflower safflower and chrysanthemum in one time, and determine the amount of adulteration. It is also valuable to other related research.