论文部分内容阅读
作者以1％乙酸冲洗雌性新西兰白兔膀胱粘膜获得其酸溶性提取物。经AU-PAGE分析表明,膀胱粘膜酸溶性提取物有十余条主蛋白带,不含已知的内源性抗菌多肽溶菌酶和防御素。琼脂糖弥散法杀菌试验显示,膀胱粘膜酸溶性提取物对致病性大肠杆菌ML-35P耐药株具杀菌活性。电泳凝胶琼脂糖弥散法杀菌试验进一步分析表明,杀菌活性与两条主蛋白带相关,命名为Rab BP-1和Rab BP-2。它们分别占膀胱粘膜提取物蛋白质总量的2.5％和1.2％。本实验的研究结果首次提示,兔膀胱粘膜存在抗菌性蛋白,这可能是膀胱粘膜杀菌这一重要防御机理的分子基础。
The authors used 1% acetic acid to flush female New Zealand white rabbits bladder mucosa to obtain its acid-soluble extract. AU-PAGE analysis showed that the bladder mucosa acid-soluble extract has more than ten major protein bands, does not contain the known endogenous antibacterial peptides lysozyme and defensin. Disinfection agarose test showed that acid-soluble bladder mucosa extract of pathogenic E.coli ML-35P resistant strains with bactericidal activity. Electrophoresis Gel Agarose Dispersion Sterilization Test Further analysis showed that the bactericidal activity was related to two major protein bands, designated Rab BP-1 and Rab BP-2. They accounted for 2.5% and 1.2%, respectively, of the total protein in the bladder mucosa extract. The results of this experiment for the first time prompted the existence of anti-bacterial protein in the bladder mucosa, which may be the molecular basis of the important defensive mechanism of bladder mucosal sterilization.