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用玻璃微电极和心脏活体灌流技术,观察到中华眼镜蛇全毒(1μg/ml)在5min内使离体大鼠窦房结起搏动作电位振幅(APA)降低48.1±SD5.8mV,最大舒张期电位(MDP)衰减-52.0±3.0mV,并最好发生“非可逆性去极化”。舒张期4相去极化速率(RP4D)减慢57.1±SD3.5 mV/s,并产生不规则的类“后电位振荡”现象,导致4相去极化和复极化的紊乱,窦性周长(P)亦延长150±SD70ms。离体大鼠乳头肌经蛇毒1μg/ml灌流,3min内即呈现剧烈的负变力效应,等长峰张力(PCF)衰减了12.5±SD 2.8mm。上述结果提示,中华眼镜蛇全毒中可能含有类“心脏毒素”组分。
Using glass microelectrode and cardiac perfusion technique, it was observed that the whole venom of Naja naja at 1μg / ml reduced the pacemaker action potential amplitude (APA) of isolated rat sinoatrial node by 48.1 ± SD5.8mV within 5min and the maximum diastolic phase The potential (MDP) is attenuated by -52.0 ± 3.0 mV, and “irreversible depolarization” preferably occurs. The diastolic 4-phase depolarization rate (RP4D) slows down by 57.1 ± SD3.5 mV / s and produces an irregular class of “post-potential oscillations” resulting in a disorder of 4-phase depolarization and repolarization, a sinusoidal (P ) Also extend 150 ± SD70ms. The isolated rat papillary muscles were perfused with snake venom 1 μg / ml for 3 min, which showed intense negative force effect. The isometric peak tension (PCF) decreased by 12.5 ± SD 2.8 mm. The above results suggest that the Chinese cobra poison may contain a class of “cardiotoxin” component.