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首次用ＣＦＵ－ＡＬＬ极限稀释液体微培养法，对１６例初诊，１４例ＣＲ期ＡＬＬ骨髓进行培养，并用８例正常，１１例再生骨髓作为ＣＦＵ－ＡＬＬ阴性对照。结果显示：初诊Ｂ系ＡＬＬ骨髓以重叠型集落伴ＣＤ（１０＋）细胞＞２０％为特点；初诊Ｔ－ＡＬＬ集落也属重叠型（ＣＤ（２＋）、ＣＤ（３＋）、ＣＤ（７＋））．而正常和再生骨髓虽形成集落，但为平展型或伴重叠型集落（ＣＤ（１０＋）＜２０％、以ＣＤ（１５＋）、ＣＤ（３３＋）细胞为主）。本文１４例ＣＲ期ＡＬＬ中，７例有重叠型集落（ＣＤ（１０＋）细胞＞２０％）；临床随访１年半，５例骨髓相继复发，１例诊断为睾丸白血病。
For the first time, CFU-ALL minimal dilution liquid micro-culture method was used to culture 16 cases of newly diagnosed and 14 CR-refractory ALL bone marrow. Eight normal and 11 cases of regenerated bone marrow were used as CFU-ALL negative control. The results showed that the newly diagnosed B-ALL bone marrow was characterized by overlapping colony with> 20% of CD (10+) cells. The newly diagnosed T-ALL colonies also belonged to overlapping type (CD (2 +), CD (3 +) and CD (7+)). Although normal and regenerated bone marrow formed colonies, they were flat or overlapped colonies (CD (10 +) <20%, mainly CD (15+) and CD (33+) cells). Of the 14 cases with CR ALL, 7 had overlapping colonies (> 20% of CD (10+) cells). One year and a half of clinical follow-up, 5 cases of bone marrow relapsed one case and one case of testicular leukemia.