目的确定特定小鼠MHCI类分子H 2d 限制性加工提呈的汉坦病毒核蛋白(NP)抗原表位。方法在计算机预测的基础上 ,以编码NP的S基因为模板 ,合成了3个相互重叠的基因片段 ,构建了以携带GFP报告基因的pEGFP N2 为载体的重组质粒 ,并进行了初步表达及鉴定。结果目的基因片段已插入质粒载体 ,并在转染的真核细胞内成功表达。结论汉坦病毒核蛋白片段能够被细胞加工提呈。 OBJECTIVE: To determine the presence of the hantavirus nucleoprotein (NP) epitopes presented by the H 2d restricted processing of specific mouse MHC class I molecules. Methods On the basis of computer prediction, three overlapping gene fragments were synthesized based on the S gene encoding NP, and a recombinant plasmid containing pEGFP N2 carrying GFP reporter gene was constructed and expressed. . Results The target gene fragment was inserted into the plasmid vector and successfully expressed in the transfected eukaryotic cells. Conclusion Hantaan virus nucleoprotein fragments can be presented by cell processing.