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为阐明TNT对肝脏损伤的机理,搞清尿中代谢物的种类,结构及含量有着十分重要的意义。将工人尿样加酸水解使代谢物游离,用苯提取液做色谱/质谱分析。鉴定出两个-氨基二硝基甲苯(4-A,2-A);两个二氨基-硝基甲苯(2,4-DA,2,6-DA)。又用化学电离源质谱/质谱法鉴定出易分解的羟氨基二硝基甲苯(4-HA)。用高效液相色谱法测定了某化工厂9名工人尿样。其含量顺序为4-A,4-HA,2-A,2,4-DA,2,6-DA。大多数尿样未检出TNT原形。4-A浓度最大值7.7mg/L,仍低于ILO规定的生物阈限值。
In order to elucidate the mechanism of liver damage caused by TNT, it is of great significance to clarify the types, structures and contents of metabolites in urine. The workers urine samples were acid-hydrolyzed metabolites free, with benzene extract chromatography / mass spectrometry. Two amino-dinitrotoluene (4-A, 2-A); two diamino-nitrotoluene (2,4-DA, 2,6-DA) were identified. Also by chemical ionization mass spectrometry / mass spectrometry identified hydroxylamine easily decomposed dinitrotoluene (4-HA). The urine samples of 9 workers in a chemical factory were determined by HPLC. The content order is 4-A, 4-HA, 2-A, 2,4-DA, 2,6-DA. Most of the urine samples were not detected TNT prototype. The maximum 4-A concentration of 7.7 mg / L remained below the bio-threshold limit set by ILO.