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目的:探讨精子DNA损伤对妊娠结局的影响以及DFI值与宫腔内人工授精(intrauterineinsemination,IUI)妊娠率的关系。方法:对186对不孕夫妇行IUI治疗,采用精子染色质结构分析法(SCSA法)检测精子DNA碎片指数(DFI),观察DFI值与IUI妊娠率的关系。结果:妊娠组患者的平均DFI值为(18.79±8.92)%,明显低于非妊娠组(29.56±9.14)%,具有统计学差异(p<0.05)。186例患者中,DFI>25%的有36例,3例临床妊娠,妊娠率为8.33%,DFI≤25%的有150例,28例临床妊娠,妊娠率为18.67%。DFI值≤25%时的临床妊娠率明显高于DFI值>25%时的临床妊娠率,具有显著性差异(p<0.01)。结论:精子DNA损伤与妊娠结局具有一定的相关性,SCSA检测的DFI值对IUI临床妊娠率具有指导意义。
Objective: To investigate the effect of sperm DNA damage on pregnancy outcome and the relationship between DFI value and intrauterine insemination (IUI) pregnancy rate. Methods: One hundred and sixty-six infertile couples underwent IUI treatment. The sperm DNA fragmentation index (DFI) was detected by the method of sperm chromatin structure analysis (SCSA), and the relationship between DFI and IUI pregnancy rate was observed. Results: The average DFI of pregnant women was (18.79 ± 8.92)%, significantly lower than that of non-pregnant women (29.56 ± 9.14%), with statistical significance (p <0.05). Of the 186 patients, 36 had DFI> 25%, 3 had clinical pregnancy, 8.33% had pregnancy, 150 had DFI ≤ 25%, and 28 had clinical pregnancy with a pregnancy rate of 18.67%. The clinical pregnancy rates at DFI values ≤25% were significantly higher than those at DFI values> 25% (p <0.01). Conclusion: There is a correlation between sperm DNA damage and pregnancy outcome. The DFI value detected by SCSA is instructive for the clinical pregnancy rate of IUI.