论文部分内容阅读
目的探讨应用C3A细胞系所构建的生物人工肝对猪急性肝衰竭动物模型的治疗效果。方法将12只中华实验猪颈外静脉注射D-氨基半乳糖以建立急性肝衰竭模型,随机分成对照组(n=6)与生物人工肝治疗组(n=6)。应用C3A细胞系构建体外生物人工肝支持系统,在给予D-氨基半乳糖24h后对治疗组进行连续36h治疗,监测治疗前后不同时间点两组动物的一般状况、生存时间和生化指标。结果对照组动物平均存活时间为(48.9±11.2)h,治疗组存活时间为(105.7±12.6)h,生物人工肝治疗组在给药后48h、72h、96h、120h等各个时间点动物生存率均大于对照组。生物人工肝治疗结束时,血清胆红素、转氨酶下降,白蛋白、凝血酶原活动度上升,与对照组比较,差异有显著性(P<0.05～P<0.01);血氨下降差异不明显(P>0.05)。结论D-氨基半乳糖猪急性肝衰竭模型能够较好的模拟临床急性肝衰竭过程,C3A细胞系生物人工肝具有一定的肝脏合成和解毒功能。
Objective To investigate the therapeutic effect of bioartificial liver constructed by C3A cell line on animal models of acute liver failure in pigs. Methods Twelve Chinese experimental pigs were injected D-galactosamine into the external jugular vein to establish a model of acute liver failure. They were randomly divided into control group (n = 6) and biological artificial liver treatment group (n = 6). C3A cell line was used to construct bioartificial liver support system in vitro. After treated with D-galactosamine for 24 hours, the treatment group was treated for 36 hours continuously. The general condition, survival time and biochemical indexes of two groups were monitored before and after treatment. Results The average survival time of the control group was (48.9 ± 11.2) h, the survival time of the treatment group was (105.7 ± 12.6) h, the survival rate of the artificial liver treatment group at various time points of 48h, 72h, 96h and 120h Are larger than the control group. At the end of biological artificial liver treatment, serum bilirubin, aminotransferase decreased, albumin and prothrombin activity increased, compared with the control group, the difference was significant (P <0.05 ~ P <0.01); blood ammonia was not significantly different (P> 0.05). Conclusion D-galactosamine in acute liver failure model of pigs can better simulate the process of clinical acute liver failure, C3A cell line artificial artificial liver has certain liver synthesis and detoxification.