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目的建立一种模拟失重性骨丢失小鼠尾悬吊模型。方法根据Morey-Holton的大鼠尾悬吊模型,加以改进,以小鼠为模式动物,进行了为期4周的小鼠尾部悬吊实验,并对小鼠的股骨进行HE染色,组织形态学观察和力学性能检测。结果在尾悬吊28 d后小鼠体重(32.11±2.79)g较对照组(37.36±2.44)g下降了14%。组织学观察显示,与对照组相比,尾悬吊小鼠股骨皮质骨内未钙化的骨基质增多,骨基质中的骨细胞较幼稚,成骨细胞少,破骨细胞多而大。股骨力学性能结果显示,尾吊小鼠股骨的刚度(P<0.05)和最大载荷(P<0.001)与对照组有明显的下降。结论尾吊28 d后的小鼠股骨发生了骨丢失,该模型可以用于模拟失重生物效应的体内实验研究。
Objective To establish a tail suspension model for simulated weightlessness bone loss in mice. Methods According to the tail suspension model of Morey-Holton rats, the mouse tail-suspension model was used to improve the mouse tail-suspension model. The tail suspension test was performed in mice for 4 weeks. HE staining and histomorphological observation were performed on the femur of mice. And mechanical properties testing. Results The body weight of mice (32.11 ± 2.79g) was 14% lower than that of the control group (37.36 ± 2.44g) after 28 days of tail suspension. Histological observation showed that compared with the control group, the non-calcified bone matrix in the femoral cortical bone of the tail-suspended mice increased, the bone cells in the bone matrix were naive, there were fewer osteoblasts and more osteoclasts. The mechanical properties of the femur showed that the femoral stiffness (P <0.05) and the maximum load (P <0.001) of the tail-suspended mice decreased significantly compared with the control group. Conclusion The bone loss occurred in mice femurs 28 days after tail suspension, and this model can be used to study the biological effects of weightlessness in vivo.