论文部分内容阅读
目的:探讨萎胃康及其拆方对慢性萎缩性胃炎(CAG)模型大鼠的治疗作用。方法:将70只Wistar大鼠随机抽取12只为正常对照组(正常组),其余58只采用多重刺激6周复制大鼠CAG模型。确定造模成功的50只随机分为5组,即模型组、萎胃康全方组(全方组)、拆方Ⅰ号组(补益组)、拆方Ⅱ号组(祛邪组)、维霉素对照组(西药组),分别ig 0.9%生理盐水(10 mL.kg-1)、萎胃康水煎液(8 g.kg-1)、拆方Ⅰ号水煎液(5.5 g.kg-1)、拆方Ⅱ号水煎液(2.5 g.kg-1)和维霉素混悬液(0.3 g.kg-1),1次/日。给药30 d后,观察对大鼠胃黏膜组织形态及血清超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量的影响。结果:全方组大鼠胃黏膜组织接近正常组。与正常组SOD(239.88±6.32)U.mL-1,MDA(3.17±0.02)μmol.L-1比较,模型组SOD(174.59±12.81)U.mL-1明显降低(P<0.01),MDA(5.14±0.15)μmol.L-1明显升高(P<0.01)。与模型组比较,各用药组大鼠血清SOD明显升高,MDA显著降低(P<0.01或P<0.05);与全方组SOD(233.91±9.03)U.mL-1,MDA(3.11±0.19)μmol.L-1比较,补益组、祛邪组大鼠血清SOD明显降低,MDA显著升高;与补益组SOD(221.58±5.71)U.mL-1比较,祛邪组大鼠血清SOD(209.89±5.27)U.mL-1活力明显降低(P<0.05)。结论:萎胃康能改善和逆转实验性萎缩性胃炎大鼠胃黏膜萎缩。其中益气养阴药物起主要作用,祛邪药物起协同作用,其机制可能与抗自由基损伤有关。
Objective: To investigate the therapeutic effect of wweweikang and its disassembled prescriptions on chronic atrophic gastritis (CAG) model rats. Methods: Twelve of 70 Wistar rats were randomly selected as the normal control group (normal group) and the other 58 rats were subjected to multiple stimulation for 6 weeks to replicate the rat CAG model. Fifty cases of successful model establishment were randomly divided into five groups: model group, wu wei kang full prescription group (total prescription group), prescription I group (benefit group), prescription removal group Ⅱ (Quxie group) The control group (western medicine group) was treated with ig 0.9% saline (10 mL.kg-1), wweweikang decoction (8 g.kg-1) -1), decoction of No. Ⅱ decoction (2.5 g.kg-1) and doxorubicin suspension (0.3 g.kg-1), 1 time / day. After administration for 30 days, the gastric mucosal histomorphology and serum superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were observed. Results: Gastric mucosa in all the rats were close to the normal group. Compared with normal group (239.88 ± 6.32) U.mL-1 and MDA (3.17 ± 0.02) μmol.L-1, SOD in model group was significantly lower (174.59 ± 12.81) U.mL- (5.14 ± 0.15) μmol.L-1 (P <0.01). Compared with the model group, serum SOD increased significantly and MDA decreased significantly in each group (P <0.01 or P <0.05) (P <0.05) .Compared with the replenishing group SOD (221.58 ± 5.71) U.mL-1, the level of serum SOD (P <0.01) 209.89 ± 5.27) U.mL-1 activity was significantly lower (P <0.05). Conclusion: Weiweikang can ameliorate and reverse gastric mucosal atrophy in experimental atrophic gastritis rats. One of the main benefits Qi Yang Yin drugs, Quxie drugs play a synergistic effect, the mechanism may be related to anti-free radical damage.