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目的观察蠕形螨体外自然死亡过程的变化,以确定虫体死亡的标准。方法采用挤压刮试法获取活螨虫,观察、记录蠕形螨活动、形态结构、内容物、颜色等的连续变化。结果活螨虫离体5h～6h,其运动由活泼到不活泼再到静止不动,而虫体内部的主要变化是颜色从暗到明以及内部肠管的显示,尤其是自始至终存在的黄色区域的变化更具代表性,末期虫体皱缩,外壳裂解。结论蠕形螨离体5h后死亡或接近死亡,离体6h虫体完全死亡,标志为活动消失,虫体皱缩或裂解,足干枯,虫体内容物减少,整体呈半透明状。
Objective To observe the changes of natural death process of Demodex mite in order to determine the standard of death of the worm. Methods The living mites were obtained by squeeze-scraping test. The changes of demodex mite activity, morphological structure, content and color were observed and recorded. The results showed that live mites separated from the body 5h ~ 6h, the movement from lively to inactive and then stationary, and the main changes inside the parasites is the color from dark to light and the internal intestine display, especially throughout the changes in the yellow area More representative, the terminal body shrinkage, shell lysis. Conclusion Demodex mites died or died nearly 5h after in vitro release. The 6h exosomes were completely dead, indicating the disappearance of activities, the shrinkage or lysis of the worms, the dryness of the feet and the reduction of the content of the parasites. The whole was translucent.