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核苷酸切除修复交叉互补（ERCC2）基因是核苷酸切除修复（NER）系统中的重要成员，被认为可能与某些肿瘤的发生和肿瘤耐药有关。由于ERCC2基因在肿瘤标本中的表达甚低，定量分析困难，故改良逆转录-聚合酶链式反应（RT－PCR），建立双管ERCC2表达的定量方法。对16株人肿瘤细胞进行定量分析，三批实验间的误差甚小。本定量方法可靠、准确，能对0．25pgDNA基因表达定量，适用于对ERCC2基因表达的生物-生理学作用作深入研究，也可被用于对其它低表达基因的定量分析。
Nucleotide excision repair cross-complementation (ERCC2) gene is an important member of the nucleotide excision repair (NER) system and is thought to be related to the occurrence of certain tumors and drug resistance in tumors. Since the expression of ERCC2 gene in tumor specimens is very low and quantitative analysis is difficult, modified reverse transcription-polymerase chain reaction (RT-PCR) was used to establish a quantitative method for dual-tube ERCC2 expression. Quantitative analysis was performed on 16 human tumor cells. The error between the three experiments was very small. This quantitative method is reliable, accurate, capable of quantifying 0.25 pg DNA gene expression, and is suitable for the in-depth study of the bio-physiological effects of ERCC2 gene expression, and can also be used for the quantitative analysis of other low-expressed genes.