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目的:了解人芽囊原虫(B.hominis)人工感染昆明小鼠后在肠道内寄生、自然病程和治疗情况,观察肠道内环境对其寄生的影响。方法:从腹泻患者粪便中分离培养人芽囊原虫,昆明小鼠分为接受免疫抑制剂处理组、非免疫抑制剂处理组和正常对照组。分别经口人工感染不同数量的人芽囊原虫。感染后第2天粪便检查观察感染情况,感染第6天处死各组2/3实验鼠,各肠段分别取肠内容物涂片镜检虫体寄生情况,观察各段肠腔内环境(pH值、肠道菌群)。观察剩余实验鼠的自然病程,2周后对未愈小鼠给予甲硝唑治疗,观察治疗效果。结果:粪便检查人芽囊原虫检出率为83.3%(75/90)。剖杀后观察pH值在6.5～7.5的肠段有虫体寄生,回盲部(pH7.04±0.16)和盲肠(pH7.1±0.15)部位多见,主要寄居在肠腔和/或附着在肠粘膜表面。各组实验鼠肠道菌群没有差别。68.4%(13/19)的剩余阳性实验鼠多在7～10天内自愈,2周后仍未愈者甲硝唑治疗100%(6/6)有效。结论:人芽囊原虫主要寄生在小鼠回盲部的肠腔和粘膜表面,肠道pH值对人芽囊原虫寄生有影响,甲硝唑治疗效果好。
OBJECTIVE: To understand the parasitic and natural course of disease and treatment of B. hominis in Kunming mice after artificial infection and to observe the effect of intestinal environment on its parasitism. Methods: Human Blastocystis sp. Was isolated and cultured from feces of patients with diarrhea. Kunming mice were divided into immunosuppressant-treated group, non-immunosuppressant-treated group and normal control group. Different numbers of human Blastocystis protozoa were respectively infected by mouth. On the second day after infection, stool examination was used to observe the infection. On the 6th day after infection, 2/3 of the mice in each group were sacrificed. The parasites of the intestine were smeared and examined for parasite status. The intestinal lumen Value, gut flora). After observing the natural course of the remaining mice, metronidazole was given to the unhealed mice 2 weeks later to observe the therapeutic effect. Results: The detection rate of stool sac was 83.3% (75/90). After the dissection, parasites were observed in the intestinal segments with pH value between 6.5 and 7.5. The ileocecal part (pH7.04 ± 0.16) and caecum (pH7.1 ± 0.15) were more common, mainly in the intestine and / or attached In the intestinal mucosal surface. There was no difference in intestinal flora between experimental groups in each group. 68.4% (13/19) of the remaining positive mice more self-healing within 7 to 10 days, 2 weeks after the treatment is not yet metronidazole 100% (6/6) effective. Conclusion: Blastocystis hominis mainly infects the intestinal lumen and mucosal surface of the ileocecal part of mice, and the intestinal pH has an effect on the parasitism of Blastocystis protozoa, and the metronidazole treatment effect is good.