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目的 探讨NR1反义寡核苷酸对局灶性脑缺血的治疗作用。方法 于大鼠大脑中动脉闭塞后2小时、2 4小时分别经侧脑室注射磷酸缓冲液 (PBS)、错义寡核苷酸 (MSODN)及反义寡核苷酸 (ASODN) ,然后在不同时间点进行神经功能缺损评分 ,术后第 5天进行Nissl染色、TTC染色及梗死体积比测定。结果 各组局灶性脑缺血的神经功能缺损评分无显著性差异 ;反义寡核苷酸治疗组的梗死体积比显著低于单纯缺血组 ;反义寡核苷酸治疗组海马各区神经元损伤轻 ,神经元丢失相对较少。结论 局灶性脑缺血后侧脑室注入NR1反义寡核苷酸 ,可以减轻缺血脑组织病理学损害 ,具有脑保护作用。
Objective To investigate the therapeutic effect of NR1 antisense oligonucleotide on focal cerebral ischemia. Methods The intracerebroventricular injection of phosphate buffered saline (PBS), missense oligonucleotide (MSODN) and antisense oligonucleotide (ASODN) were performed 2 hours and 24 hours after the occlusion of the middle cerebral artery in rats. Neurological deficit score was measured at the time point. Nissl staining, TTC staining and infarction volume ratio were performed on the 5th postoperative day. Results There was no significant difference in the scores of neurological deficit in each group of focal cerebral ischemia; the volume of infarction in antisense oligonucleotide group was significantly lower than that in ischemia group; in the antisense oligonucleotide group, Light damage, neuronal loss is relatively small. Conclusion The intracerebroventricular injection of NR1 antisense oligonucleotide after focal cerebral ischemia can alleviate the pathological damage of ischemic brain tissue and has neuroprotective effects.