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目的探讨以AEAPS及葡聚糖共修饰超顺磁纳米颗粒(superparamagnetic nanoparticles,SPION)的制备方法,并检测其物理和磁学性质。方法共沉淀一步法制备葡聚糖包被的四氧化三铁纳米颗粒,然后在通氮、60℃、强力机械搅拌的条件下加入AEAPS,得到AEAPS与葡聚糖共修饰的SPION。采用透射电镜测量其大小及分布,用振动样品磁强计检测磁化率等参数,用普鲁士蓝染色的方法判断AEAPS/葡聚糖-SPION是否与抗体连接。结果所得样品核心为四氧化三铁晶体,核心粒径不超过20nm,包被葡聚糖后整体颗粒直径不超过25nm,质量饱和磁场强度为67.36488emu/g Fe,AEAPS/葡聚糖-SPION与抗体结合,可对特异性抗原产生抗原-抗体结合,普鲁士蓝染色结果为阳性。结论制备的样品具有粒径小,分散性好,超顺磁性等优点,能与单克隆抗体有效结合。
Objective To investigate the preparation of superparamagnetic nanoparticles (SPION) co-modified with AEAPS and dextran, and to examine its physical and magnetic properties. Methods Dextran-coated ferroferric oxide nanoparticles were prepared by coprecipitation in one-step method. AEAPS was co-modified with dextran by adding AEAPS under strong mechanical stirring at 60 ℃. Transmission electron microscopy was used to measure the size and distribution. Magnetic susceptibility was measured with a vibrating sample magnetometer. Prussian blue staining was used to determine whether the AEAPS / dextran-SPION was linked to the antibody. Results The core of the obtained sample was ferric oxide crystals with a core particle size of no more than 20 nm. The overall particle diameter after coating with dextran was no more than 25 nm and the mass saturation magnetic field intensity was 67.36488 emu / g Fe. AEAPS / dextran-SPION and Antibody binding, antigen-antibody binding to specific antigens, Prussian blue staining was positive. Conclusion The prepared sample has the advantages of small particle size, good dispersion and superparamagnetism, and can be effectively combined with monoclonal antibody.