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目的通过观察大鼠脑挫裂伤后脑水肿的磁共振显像(MRI)动态变化,分析中药脑疏宁对脑水肿的干预效果。方法SD雄性大鼠114只,随机分为脑疏宁组、模型组和假手术组。采用自由落体撞击法建立大鼠脑挫裂伤模型,术后连续动态观察T_2加权像(T_2WI)病灶区信号强度(SI)与体积变化,采用干湿重法测定脑组织含水量。结果外伤后术侧大脑半球见T_2WI高信号改变,1 d时信号强度最高、体积最大,至7 d下降至最低点,与脑组织含水量的变化一致。脑疏宁组各时间点T_2WI信号强度、水肿体积及脑含水量均显著小于模型组(P<0.05),术后7 d水肿吸收率显著高于模型组(P<0.005)。结论无创性MRI检查能够对大鼠脑外伤及用药后脑水肿程度作出定量分析,是评价药物疗效的可靠手段;中药脑疏宁能够促进大鼠脑挫裂伤后脑水肿的吸收。
Objective To investigate the effect of brain edema with brain sclerotherapy by observing the dynamic changes of magnetic resonance imaging (MRI) of brain edema after cerebral contusion in rats. Methods 114 SD male rats were randomly divided into acupuncture group, model group and sham operation group. A rat model of brain contusion was established by the free-fall impact method. The signal intensity (SI) and volume changes of the lesion area were continuously observed on a T_2 weighted image (T_2WI). The water content of the brain tissue was determined by the wet weight method. Results After the trauma, the signal of T_2WI was changed in the cerebral hemisphere after trauma. The signal intensity was the highest and the volume was the largest at 1 d. It decreased to the lowest point at 7 d, which was consistent with the changes of brain tissue water content. The signal intensity, edema volume, and brain water content of T2WI at the brain stalk group were significantly lower than those in the model group (P<0.05). The edema absorption rate at the 7th day after operation was significantly higher than that of the model group (P<0.005). Conclusion The noninvasive MRI examination can quantitatively analyze the degree of brain trauma and brain edema after drug administration in rats, and it is a reliable method to evaluate the efficacy of the drug. The Chinese medicine Naoshun Ning can promote the absorption of cerebral edema after cerebral contusion in rats.