本文用图像分析仪对初次和再次叮咬宿主兔后的中华硬蜱中肠消化细胞进行了ＤＮＡ和形态测量学分析。结果表明；蜱血餐后其中肠消化细胞ＤＮＡ增加，约第５天达高峰。初次叮咬组吸血后ＤＮＡ倍体数增加显著，第五天ＤＮＡ平均倍体数达７０６，峰值达１２Ｃ，≥５Ｃ细胞达４４２％。且随叮咬吸血时间延长，中肠增粗，直径增大；肠壁增厚，肠壁横截面面积增大；消化细胞增多，消化细胞截面数增加；消化细胞增大，消化细胞平均截面面积增大；而消化细胞数面密度下降，消化细胞核的体密度和表面积密度下降。这些结果均是对吸入血液进行消化和储存营养物质的正常反应。当中华硬蜱叮咬经初次感染后的宿主兔时，ＤＮＡ倍体数、横切面肠壁面积、消化细胞截面数、消化细胞平均截面面积等增加的幅度不如初次叮咬组，而消化细胞核的体密度和表面积密度下降更显著，提示由于初次叮咬使宿主产生了较强的免疫力而使中肠消化细胞破坏、脱落或抑制增殖而出现吸血量下降，吸血后的上述各种正常反应降低 In this paper, image analysis was used to analyze the DNA and morphology of intestinal metaphase cells of Ixodes sinensis after initial and again bite host rabbits. The results showed that the amount of digestive cell DNA increased after the meal blood meal reached its peak on the 5th day. After the first bite group, DNA ploidy increased significantly. On the fifth day, the average DNA multiploid number reached 706, peaked at 12 ℃, and ≥5C reached 442%. And with the bite vampire time, the thickening of the midgut, the diameter increased; thickening of the intestinal wall, the intestinal wall area increased; digestive cells increased, the number of digestive cells increased; digestive cells increased, the average cross-sectional area of ​​digestive cells increased Large; while the number of digestible cells decreased surface density, density and density of the digestive cell nucleus decreased. These results are normal reactions to the blood that is drawn in to digest and store nutrients. When Ixodes sinensis bites the host rabbits after initial infection, the DNA ploidy, cross-sectional area of ​​intestinal wall, the number of digested cells and the average cross-sectional area of ​​digested cells did not increase as much as the initial bite group, while the density of digested nuclei And the surface area density decreased more significantly, suggesting that due to the initial bite the host has a strong immune system in the midgut digestive cells destruction, shedding or inhibition of proliferation and decline in the amount of blood-sucking, vomiting after the above-mentioned various normal reactions decreased