试验通过高温、CO2加富下对温室嫁接黄瓜的形态特征、净光合速率、Rubisco羧化酶日变化及其不同时间内Rubisco羧化酶变化进行测定分析,旨在为夏秋季温室黄瓜高产优质栽培提供理论依据。结果表明,高温、CO2加富下温室嫁接黄瓜植株生长旺盛,株高、茎粗、叶面积、叶片厚度均明显高于其他处理。经过高温、CO2处理后的温室嫁接黄瓜,其Rubisco羧化酶活性日变化呈“双峰”曲线,第一个峰出现在上午9:00前后,第二个峰在下午15:00左右,并在两峰间13:00达到低谷,其日变化始终高于其他处理。常温条件下,CO2加富后在较低温度时(9:00),会抑制Rubisco羧化酶的活性,而温度升高后(13:00和15:00),可以提高Rubisco羧化酶的活性。单纯的高温会抑制Rubi-sco羧化酶的活性,而CO2加富后,可以提高Rubisco羧化酶的活性,显著地高于对照与常温、CO2处理。高温、CO2处理温室嫁接黄瓜净光合速率的变化与其Rubisco羧化酶活性的变化呈显著的正相关关系。CO2加富可以增加温室嫁接黄瓜叶片的净光合速率,即使9:00羧化酶的活性较低时,净光合速率也高于对照和单纯高温处理。CO2加富配合高温可以更有效地提高温室嫁接黄瓜的光合作用,进而促进了温室嫁接黄瓜植株的生长。 The experiment analyzed the morphological characteristics, net photosynthetic rate, Rubisco carboxylase diurnal variation of Rubisco carboxylase and the changes of Rubisco carboxylase in different time under high temperature and CO2 enrichment. The aim was to provide high-yield and high-quality cucumber cultivation in summer and autumn, Provide a theoretical basis. The results showed that under the condition of high temperature and CO2 enrichment, the grafted cucumber plants grew vigorously and the plant height, stem diameter, leaf area and leaf thickness were significantly higher than those of other treatments. After high temperature, CO2 treatment of greenhouse grafted cucumber, Rubisco carboxylase activity diurnal changes were “double peak ” curve, the first peak appeared before 9:00 am, the second peak around 15:00 in the afternoon , And reached the lowest point at 13:00 between the two peaks. The daily change was always higher than other treatments. At room temperature, Rubisco carboxylase activity was inhibited at lower temperature (9:00) after CO2 enrichment, while Rubisco carboxylase activity was increased at elevated temperature (13:00 and 15:00) active. Pure high temperature inhibited the activity of Rubi-sco carboxylase, and CO2 enrichment increased the activity of Rubisco carboxylase, which was significantly higher than that of control and normal temperature and CO2 treatment. The changes of net photosynthetic rate and the changes of Rubisco carboxylase activity in grafted cucumber under high temperature and CO2 treatment were significantly positively correlated. CO2 enrichment can increase the net photosynthetic rate of greenhouse cucumber leaves grafted, even if 9:00 carboxylase activity is lower, the net photosynthetic rate is also higher than the control and simple high temperature treatment. CO2 enrichment with high temperature can be more effective in greenhouse greenhouse grafted cucumber photosynthesis, thus promoting the greenhouse cucumber plant growth.