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[Objective] To research the protective effects of FRUCTUS SCHISANDRAE CHINENSIS A, B and C on the oxidative damage of HaCat cells, so as to provide foundations for the development of FRUCTUS SCHISANDRAE CHINENSIS anti-aging products. [Method] Aging model of the oxidative damage of HaCat cells induced by H2O2 was established. And proliferation of HaCat cells was detected by MTT assay. [Result] The group of 50 μmol/L H2O2 showed no inhibitory effects on cells; but 100 and 200 μmol/L groups had significant inhibitory effects. The survival rate of cells in H2O2 group was (71.667±0.862)%. The survival rates of cells in 100 and 200 μmol/L FRUCTUS SCHISANDRAE CHINENSIS A groups were (76.400±0.656)% and (82.367±0.666)%, respectively. The survival rates of cells in 100 and 200 μmol/L FRUCTUS SCHISANDRAE CHINENSIS B groups were (86.300±0.721)% and (88.900±0.985)% , respectively. The survival rates of cells in 100 and 200 μmol/L FRUCTUS SCHISANDRAE CHINENSIS C groups were (86.767±0.551)% and (88.067±0.873)% , respectively. [Conclusion] FRUCTUS SCHISANDRAE CHINENSIS A, B and C all had certain protective effects on the oxidative damage of HaCat cells induced by H2O2. And FRUCTUS SCHISANDRAE CHINENSIS B and C showed relatively better protection effects.
[Objective] To research the protective effects of FRUCTUS SCHISANDRAE CHINENSIS A, B and C on the oxidative damage of HaCat cells, so as to provide foundations for the development of FRUCTUS SCHISANDRAE CHINENSIS anti-aging products. [Method] Aging model of the oxidative Damage of HaCat cells induced by H2O2 was established. And proliferation of HaCat cells was detected by MTT assay. [Result] The group of 50 μmol / L H2O2 showed no inhibitory effects on cells; but 100 and 200 μmol / L groups had significant inhibitory The survival rates of cells in H2O2 group was (71.667 ± 0.862)%. The survival rates of cells in 100 and 200 μmol / L FRUCTUS SCHISANDRAE CHINENSIS A groups were (76.400 ± 0.656)% and (82.367 ± 0.666)%, respectively. The survival rates of cells in 100 and 200 μmol / L FRUCTUS SCHISANDRAE CHINENSIS B groups were (86.300 ± 0.721)% and (88.900 ± 0.985)%, respectively. The survival rates of cells in 100 and 200 μmol / L FRUCTUS SCHISANDRAE CHINENSIS C groups were (86.767 ± 0.551 ) and% (88.067 ± 0.873)%, respectively. [Conclusions] FRUCTUS SCHISANDRAE CHINENSIS A, B and C all had certain protective effects on the oxidative damage of HaCat cells induced by H2O2. And FRUCTUS SCHISANDRAE CHINENSIS B and C < effects.