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首次应用单扩溶血试验(SRH)检测包虫病人抗体,试验最适条件为:使用纯化人包虫抗原20μg/ml浓度致敏红细胞60分钟,加1:5补体,放37℃温育8小时,溶血圈直径≥5mm判定为阳性。各种来源血清标本试验结果:26例经手术及临床证实的包虫病人中抗体检出率达84.62％,各种其他病人,正常人合计假阳性率为8.11％。阳性包虫病人中,不同寄生部位在敏感性上没有显著差异。试验表明,SRH操作简便,具有较高的敏感性和特异性等优点。SRH能否作为包虫手术后判定预后的一个手段是值得进一步研究的。
The first application of single expansion hemolysis test (SRH) detection of hydatid antibody, the optimum conditions for the experiment: using purified human hydatid antigen 20μg / ml concentration of sensitized erythrocytes for 60 minutes, add 1: 5 complement, put 37 ℃ incubated 8 Hour, hemolytic circle diameter ≥ 5mm, judged as positive. Serum samples from a variety of sources test results: 26 cases of hydatid disease confirmed by surgery and clinically detected antibody was 84.62%, a variety of other patients, normal false positive rate of 8.11%. Among the positive hydatid patients, there was no significant difference in the sensitivity of different parasitic sites. Experiments show that, SRH is easy to operate, with high sensitivity and specificity. Whether SRH can be used as a tool to determine the prognosis of hydatid disease is worth further study.