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在饲料中添加不同比例的苦地胆内酯,饲喂斑马鱼成鱼14 d后,采用创伤感染法构建斑马鱼嗜水气单胞菌感染模型,研究苦地胆内酯对嗜水气单胞菌感染斑马鱼的保护效果。结果显示:饲喂含苦地胆内酯0.4%、0.8%、1.6%饲料的斑马鱼,其存活率显著高于阳性对照组(p值<0.05);0.8%和1.6%的苦地胆内酯对斑马鱼的保护率分别为(73.91±6.52)%和(73.91±11.30)%,与0.1%恩诺沙星保护率(76.08±7.53)%相比无显著差异(p值>0.05)。一定剂量的苦地胆内酯对嗜水气单胞菌感染斑马鱼具有保护效果。
Addition of different ratios of bitter bilobalide to the feed, feeding zebrafish adult fish for 14 days, wound infection method to build zebrafish Aeromonas hydrophila infection model to study the bitter bile lactone on water vapor single Protection of zebrafish by the fungal infection. The results showed that the survival rate of zebrafish fed with 0.4%, 0.8% and 1.6% of bile lactone was significantly higher than that of positive control group (p <0.05); 0.8% and 1.6% (73.91 ± 6.52)% and (73.91 ± 11.30)% for zebrafish, respectively. There was no significant difference (p> 0.05) between the protection rate and the protection rate of 0.1% enrofloxacin (76.08 ± 7.53)%. A certain dose of bitter bile lactone has a protective effect on Aeromonas hydrophila infection of zebrafish.