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[目的]探讨PM2.5对人永生化角质形成细胞(HaCaT)的损伤作用及NF-κB抑制剂PDTC对PM2.5致HaCaT细胞损伤作用的影响.[方法]将HaCaT细胞分别PM2.5、PDTC、PM2.5+PDTC共孵育24h,同时设不加任何处理因素的正常组.24h后收集细胞,测定细胞内丙二醛(MDA)含量、活性氧(ROS)水平、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-px)的活性以及NF-κBp65的蛋白表达情况.[结果]HaCaT细胞的存活率随PM2.5浓度的增加而下降,浓度为200、400、800μg/mL组的细胞存活率明显低于正常组(P<0.05).PM2.5组细胞中MDA含量、ROS水平和NF-κBp65蛋白表达水平显著高于正常组、PDTC组和PM2.5+PDTC组(P<0.05),SOD、GSH-Px的活性显著低于上述各组(P<0.05).PM2.5+PDTC组细胞中MDA含量、ROS水平和NF-κBp65蛋白表达水平显著高于正常组和PDTC组.[结论]PM2.5能够降低HaCaT细胞的存活率.PM2.5能够对HaCaT细胞造成氧化损伤,而PDTC能够缓解这种损伤作用.应用NF-κB抑制剂PDTC可以特异性抑制NF-κB的表达,在一定程度上减轻PM2.5对HaCaT细胞的损伤作用.“,”[Objective]To explore the effects of PM 2.5 on HaCaT cells and whether or not PDTC will alleviate the adverse effects caused by PM 2.5.[Methods]HaCaT cells were co-cultured with PM2.5,PDTC,PM2.5+ PDTC for 24 hours,respectively.HaCaT cells without any treatment were set as control.The content of malondialdehyde (MDA),reactive oxygen species(ROS)level,the activities of superoxide dismutase(SOD)and glutathione peroxi-dase(GSH-Px)_and the expression of NF-kB in the cells from different groups were measured and compared.[Re-sults]Cell viability decreased with the increasing of PM 2.5 concentrations,the cell viabilities of 200,400 and 800 μg/ml groups were significantly lower than the normal group(P <0.05).Compared with PM2.5 group,the MDA con-tents,the level of ROS and the expression of NF-kB of the cells treated with PM2.5+ PDTC,or PDTC,or vehicle treated cells were significantly lower while the activities of SOD and GSH-Px were significantly higher(P <0.05). Furthermore,the levels of MDA,ROS and NF-κB p65 protein in PM2.5 + PDTC group were significantly higher than those in control group and PDTC group [Conclusion]PM2.5 was able to decrease the cell viability and cause oxi-dative damage to HaCaT cells while PDTC can alleviate the damages to certain extent.