[Objective] The differences among the ginseng germplasm resources in five different producing regions of Jilin Province were analyzed,so as to provide theoretical basis for the introduction of ginseng from other places.[Method] By using simple sequence repeat(SSR),nine ginseng materials of five districts in Jilin were used for genomic polymorphic analysis.The dissimilarity of coefficient was calculated by professional software DPS 9.50.And the dendrogram was made by using Nei-Li formula and UPGMA.[Result] 16 pairs of polymorphic primers were selected from 25 pairs of primers for the amplification.181 bands were amplified and 117 bands were polymorphic,with the polymorphic percentage of 64.7%.[Conclusion] There was much genetic diversity at the molecular level among the germplasm resources of Panax ginseng actually.The differences between the tested ginseng variteal types were determined by the genetic factors.Furthermore,it was also affected by environmental factors. [Objective] The differences among the ginseng germplasm resources in five different producing regions of Jilin Province were analyzed, so as to provide theoretical basis for the introduction of ginseng from other places. [Method] By using simple sequence repeat (SSR), nine ginseng materials of five districts in Jilin were used for genomic polymorphic analysis. The dissimilarity of coefficient was calculated by professional software DPS 9.50. End the dendrogram was made by using Nei-Li formula and UPGMA. [Result] 16 pairs of polymorphic primers were selected from 25 pairs of primers for the amplification.181 bands were amplified and 117 bands were polymorphic, with the polymorphic percentage of 64.7%. [Conclusion] There was much genetic diversity at the molecular level among the germplasm resources of Panax ginseng actually. These differences between the tested ginseng variteal types were determined by the genetic factors .Furthermore, it was also affected by environmental factors.