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目的:研究肺癌组织及癌旁组织中血管内皮生长因子C(vascular endothelial growth factor C,VEGF-C)及其受体mRNA的表达,分析其在肿瘤转移中的作用。方法:采用RT-PCR法分析36例新鲜肺癌组织及癌旁组织标本中VEGF-C/KDR/Flt-4mRNA的表达,并统计分析它与临床病理特点之间的关系。结果:癌旁组织(PT)中VEGF-C mRNA表达与Flt-4mRNA的表达呈显著正相关(r=0.523,P=0.005 5)。肿瘤组织及癌旁组织中单一的VEGF-C、KDR或Flt-4 mRNA表达与肿瘤转移无明显相关;COMVEGF- C-3与淋巴结转移(r=0.580 5,P=0.000 8)及临床病理分期(r=0.505 7,P=0.0064)呈明显正相关。结论: VEGF-C及其受体在肺癌组织及瘤旁组织中高表达,在肿瘤转移尤其是淋巴转移中起重要作用, COMVEGF-C-3是反映患者淋巴转移和预后的良好指标。
OBJECTIVE: To study the expression of vascular endothelial growth factor C (VEGF-C) and its receptor mRNA in lung cancer tissues and paracancerous tissues, and to analyze its role in tumor metastasis. Methods: The expression of VEGF-C / KDR / Flt-4 mRNA was detected by RT-PCR in 36 specimens of fresh lung cancer tissues and adjacent normal tissues. The relationship between VEGF-C / KDR / Flt-4 mRNA and clinicopathological features was statistically analyzed. Results: There was a significant positive correlation between the expression of VEGF-C mRNA and the expression of Flt-4 mRNA in the adjacent tissue (r = 0.523, P = 0.005 5). The expression of VEGF-C, KDR or Flt-4 mRNA was not correlated with tumor metastasis in tumor tissues and paracancerous tissues. There was no significant correlation between the expression of VEGF-C, KDR or Flt-4 mRNA in tumor tissues and adjacent tissues (r = 0.505 7, P = 0.0064) showed a significant positive correlation. CONCLUSION: VEGF-C and its receptor are highly expressed in lung cancer tissues and adjacent tissues and play an important role in tumor metastasis, especially lymph node metastasis. COMVEGF-C-3 is a good indicator of lymph node metastasis and prognosis.