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用间接免疫荧光技术和逆行示踪法探讨了杏仁核的外侧核和基底外侧核神经肽 Y免疫阳性纤维的起始。结果证明 :将逆行示踪物荧光金注射到杏仁核的外侧、基底外侧核之后 ,在同侧的杏仁前区出现了许多荧光标记细胞 ;免疫荧光的染色显示部分细胞同时呈神经肽 Y免疫反应阳性 ;损毁杏仁核前区引起同侧杏仁核的外侧核和基底外侧核神经肽 Y纤维的减少。这些发现提示 ,杏仁前区的神经肽 Y免疫阳性细胞投射到同侧杏仁核的外侧核和基底外侧核。此外 ,现在的研究显示 ,出现于杏仁核的外侧核和基底外侧核的神经肽 Y免疫阳性神经元是内在性的 ,因为损毁杏仁前区后在以上的部位仍可观察到相当数量的神经肽 Y免疫阳性纤维 ;并且横断两条主要的杏仁核传出通路 -终纹和腹侧杏仁核传出通路 ,未能引起神经肽 Y免疫阳性物质在轴突近侧断端的蓄积
Indirect immunofluorescence and retrograde tracing methods were used to investigate the onset of neuropeptide Y immunoreactive fibers in the lateral and basolateral nuclei of the amygdala. The results showed that fluorescent retrograde tracer was injected into the outer side of the amygdala and basolateral nucleus, and many fluorescently labeled cells appeared in the ipsilateral apical amygdala. Immunofluorescence staining showed that some cells showed neuropeptide Y immunoreactivity Positive; destruction of the amygdala precore zone caused by ipsilateral amygdala and basal lateral hypothalamic neuropeptide Y fibers decreased. These findings suggest that neuropeptide Y immunoreactive cells in the anterior almond region project to the lateral and basolateral nuclei of the ipsilateral amygdala. In addition, current studies have shown that neuropeptide Y immunoreactive neurons appearing in the lateral and basolateral nuclei of the amygdala are intrinsically as significant amounts of neuropeptides are still observed in the above sites after destruction of the amygdala Y-immunoreactive fibers; and traversing the two major amygdala-efferent pathways-the telomere and ventral amygdala, failed to induce the accumulation of neuropeptide Y-immunopositive substance at the proximal stump of axons