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目的采用微弧氧化(micro-arc oxidation,MAO)技术,在纯钛表面制备含锌(Zn)活性涂层,并评价其对成骨细胞活性的影响。方法本实验于2009年10月至2010年10月在中国医科大学附属口腔医院中心实验室进行。以机械抛光纯钛试样作为对照组(CP组);经MAO技术处理的纯钛涂层试样根据钙(Ca)含量不同分为高钙组(H-Ca组)、低钙组(L-Ca组),以及在低钙组的涂层中加入锌元素的低钙加锌组(L-Ca-Zn组)。每组32个试件。将4组试样材料与MG63细胞复合培养,利用扫描电子显微镜(SEM)观察、噻唑蓝(MTT)比色试验、碱性磷酸酶(ALP)活性测定及酶联免疫吸附试验(ELISA)等方法,评价材料对成骨细胞黏附、增殖、分化及矿化的影响。所得数据采用SPSS13.0软件进行单因素方差分析(α=0.05)。结果在实验检测期间,H-Ca组试样对成骨细胞的生长增殖、分化、矿化作用均优于L-Ca组,差异有统计学意义(P<0.05)。比较L-Ca-Zn组与H-Ca组可以看出,在实验检测初期H-Ca组试样对成骨细胞的生长增殖、分化、矿化作用优于L-Ca-Zn组;随着观察时间的延长,L-Ca-Zn组试样细胞的增殖水平和ALP活性与H-Ca组间差异无统计学意义(P>0.05)。比较L-Ca-Zn组和L-Ca组可以看出,在实验检测初期L-Ca-Zn组试样对成骨细胞的作用与L-Ca组差异无统计学意义(P>0.05);随着观察时间的延长,L-Ca-Zn组试样显示出较L-Ca组试样具有更好的提高成骨细胞活性的作用(P<0.05)。结论采用MAO技术制备的含锌活性涂层纯钛可在一定程度上促进成骨细胞黏附、增殖、分化及矿化,具有良好的生物相容性。
OBJECTIVE: To prepare zinc (Zn) -containing coatings on pure titanium by micro-arc oxidation (MAO) technique and evaluate its effect on the activity of osteoblasts. Methods The experiment was performed in the Central Laboratory of Stomatology Hospital, China Medical University from October 2009 to October 2010. (CP group). The pure titanium coating samples treated with MAO technique were divided into high calcium group (H-Ca group) and low calcium group (L) according to the content of calcium (Ca) -Ca group), and the low-calcium plus zinc group (L-Ca-Zn group) containing the zinc element in the coating of the low-calcium group. 32 specimens per group. Four groups of samples were cultured with MG63 cells. The morphology of the cells was observed by scanning electron microscopy (SEM), MTT colorimetric assay, alkaline phosphatase (ALP) activity assay and enzyme-linked immunosorbent assay (ELISA) , To evaluate the effect of materials on osteoblast adhesion, proliferation, differentiation and mineralization. The data obtained using SPSS13.0 software for one-way analysis of variance (α = 0.05). Results During the experiment, the proliferation, differentiation and mineralization of osteoblasts in H-Ca group were better than L-Ca group (P <0.05). Compared with L-Ca-Zn group and H-Ca group, it can be seen that in the early stage of experiment, the growth, differentiation and mineralization of osteoblasts in H-Ca group were better than L-Ca-Zn group There was no significant difference between L-Ca-Zn group and H-Ca group in proliferation and ALP activity (P> 0.05). Compared with L-Ca-Zn group and L-Ca group, it can be seen that the effect of L-Ca-Zn group on osteoblasts at the beginning of the experiment was not statistically different from L-Ca group (P> 0.05). With the extension of observation time, L-Ca-Zn group showed better osteoblast activity than L-Ca group (P <0.05). Conclusion Pure titanium with active coating prepared by MAO technique can promote the adherence, proliferation, differentiation and mineralization of osteoblasts to a certain extent and has good biocompatibility.