论文部分内容阅读
【目的】建立鸡血藤药材的高效液相(HPLC)指纹图谱和芒柄花素含量测定的方法,评价不同产地鸡血藤的质量。【方法】采用反相高效液相色谱法(RP-HPLC)建立鸡血藤指纹图谱,以Feini Gen Red Classical AQ-C18(4.6 mm×250 mm,5μm)为色谱柱,乙腈—体积分数0.1%冰醋酸溶液进行梯度洗脱,检测波长260 nm;芒柄花素含量测定以Acclaim TM 120-C18柱(4.6 mm×250 mm,5μm)为色谱柱,乙腈—水等度洗脱,检测波长为254 nm,流速1.0 m L/min,柱温25℃。【结果】对24批来自不同产地的鸡血藤色谱指纹图谱进行评价,建立了鸡血藤标准指纹图谱,以芒柄花素为参照峰,确定了13个共有峰,并应用高效液相色谱—二极管阵列检测器(HPLC-DAD)法测定样品中芒柄花素的含量。不同产地鸡血藤药材指纹图谱相似度和芒柄花素含量差异较大。【结论】本研究所建立的方法简便准确、灵敏度高、重复性好,可作为鸡血藤的药材质量控制方法。
【Objective】 The objective of this study was to establish HPLC fingerprinting and determination of actin in Cordyceps militaris, and to evaluate the quality of Millettia japonica. 【Method】 The fingerprint of Millettia japonica was established by reversed-phase high performance liquid chromatography (RP-HPLC). The chromatographic column was Feini Gen Red Classical AQ-C18 (4.6 mm × 250 mm, 5 μm) Glacial acetic acid, the detection wavelength was 260 nm. The determination of formononin was performed on an Acclaim TM 120-C18 column (4.6 mm × 250 mm, 5 μm) with isocratic elution of acetonitrile-water. The detection wavelength was 254 nm, flow rate 1.0 m L / min, column temperature 25 ℃. 【Result】 Twenty-four batch fingerprints of Millettia splendens from different habitats were evaluated, and the standard fingerprints of Millettia radix sp. Were established. Thirteen common peaks were identified with the base of the formononetin, and the common peaks were identified by high performance liquid chromatography Determination of the content of formononetin in sample by diode array detector (HPLC-DAD). Fingerprints of Jixiao vine in different areas have similarities in fingerprints and large differences in the content of actin. 【Conclusion】 The method established in this study is simple and accurate, with high sensitivity and good reproducibility. It can be used as a quality control method for Jixiao vine.