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目的：无胶筛分毛细管电泳与琼脂糖电泳两种方法检测ＡｐｏＢ１００基因点突变的比较。方法：聚合酶链反应（ＰＣＲ）扩增ＡｐｏＢ１００基因的２６号外显子，ＸｂａⅠ酶切后，分别用常规琼脂糖电泳和无胶筛分毛细管电泳对比检测了４３例标本。结果和结论：毛细管电泳的突变检出率明显高于琼脂糖凝胶电泳，是一种研究基因突变与高脂血症关系更有效的分析手段。
OBJECTIVE: To compare the ApoB100 point mutation detection by gel filtration capillary electrophoresis and agarose gel electrophoresis. Methods: The exon 26 of ApoB100 gene was amplified by polymerase chain reaction (PCR). After Xba Ⅰ digestion, 43 samples were detected by conventional agarose gel electrophoresis and gel filtration - free capillary electrophoresis respectively. RESULTS AND CONCLUSION: The detection rate of mutation in capillary electrophoresis is significantly higher than that of agarose gel electrophoresis. It is a more effective method to study the relationship between gene mutation and hyperlipidemia.