亲和层析以其特异性高、迅速、操作简便、可在温和条件下进行等优点,已成为不可缺少的一项分子生物学技术。多年来,经典的方法是用CNBr进行活化和偶联的,尽管这是已被人们接受的成熟方法,但也有其不足之处,如毒性大、操作条件要求严格、不稳定等。近年来,科学工作者开始探索, Affinity chromatography with its high specificity, rapid, easy to operate, can be carried out under mild conditions and other advantages, has become an indispensable molecular biology technology. For many years, the classical method was activated and coupled with CNBr. Although this is a well-established and accepted method, it has its disadvantages, such as high toxicity, strict operating conditions and instability. In recent years, scientists began to explore,