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[目的]研究母鼠金雀异黄素干预对雄性子代大鼠肥胖的影响。[方法]选用性成熟雌性大鼠,受孕后在孕期及哺乳期间均给予不同剂量的金雀异黄素干预,并设立对照组。干预组母鼠采用添加金雀异黄素的饲料喂饲,剂量分别为3 600、1 200、400 mg/kg。子鼠断乳后采用未添加金雀异黄素的饲料喂饲,直至8周末。实验期间每周观察雄性子鼠的体重;实验结束后测定大鼠身长计算Lee’s指数,留取脂肪组织,计算脂肪脏器系数,分离血清进行血脂、血糖、胰岛素和炎性因子的检测。[结果]实验8周末,与对照组相比:3 600 mg/kg组子鼠体重降低(P=0.028)、高密度脂蛋白升高(P=0.038),3 600 mg/kg组和1 200 mg/kg子鼠组Lee’s指数、总脂肪湿重、血糖水平降低(P=0.031,P=0.049;P=0.009,P=0.016;P=0.033,P=0.026)。实验组子鼠胰岛素、炎性因子未发生明显变化。[结论]孕期及哺乳期金雀异黄素干预,可以降低雄性子鼠肥胖的发生。
[Objective] To investigate the effects of genistein on the obesity of male offspring rats. [Method] The sexual maturity female rats were selected and intervened by different doses of genistein during pregnancy and lactation after conception. A control group was established. The intervention group rats were fed genistein supplemented with dosages of 3 600,1 200 and 400 mg / kg, respectively. The offspring were fed with genistein-free diet until the weekends. The body weight of the male offspring was observed every week during the experiment. After the experiment, the length of the rats was measured to calculate the Lee’s index. The fat tissue was collected to calculate the fat organ coefficient. The serum was separated for the detection of the blood lipids, blood glucose, insulin and inflammatory factors. [Results] At the end of the 8th week, compared with the control group, body weight of 3 600 mg / kg group rats decreased (P = 0.028), high density lipoprotein increased (P = 0.038), 3600 mg / kg group Lee’s index, total fat wet weight, and blood glucose levels were lower in the mg / kg group (P = 0.031; P = 0.049; P = 0.009, P = 0.016; P = 0.033, P = 0.026). Experimental group rats insulin, inflammatory factors did not change significantly. [Conclusion] Genistein intervention during pregnancy and lactation can reduce the incidence of obesity in male rats.