论文部分内容阅读
由花青素合成代谢形成的紫色柱头性状在包括长雄野生稻在内的AA基因组野生稻中较为普遍.为研究长雄野生稻中的紫色柱头性状,以具无色柱头的亚洲栽培稻品种RD23为轮回亲本与紫色柱头的长雄野生稻进行回交,经胚挽救和多代连续选择,获得3个柱头颜色有分离的BC6F1定位群体.这些群体中,柱头颜色均适合1(紫色)∶1(无色)的分离比例,表明紫色柱头性状受一对显性核基因控制.通过微卫星标记分析,将控制紫色柱头的基因定位在水稻第6染色体上,距标记RM253,RM111和RM6917分别为2.5,0cM和4.4cM.对比已发表的紫色柱头基因座位,它可能与来自亚洲栽培稻的Ps-4(t)基因等位,所以暂命名为Ps-4(t).
The purple stigma trait formed by anthocyanin biosynthesis is common in AA genomic wild rice, including long male wild rice.In order to study the purple stigma trait in Changxiong wild rice, the Asian stigma cultivar with colorless stigma RD23 was backcrossed with the purple stigma of the male parent Oryza sativa L. The three BC6F1 loci with stigma color separation were obtained by embryo rescue and multiple generations of continuous selection.The stigma color of these populations were all suitable for 1 (purple): 1 (colorless), indicating that purple stigma traits are controlled by a pair of dominant nuclear genes.Microsatellite markers were used to locate the genes controlling the purple stigma on chromosome 6 of rice from markers RM253, RM111 and RM6917, respectively 2.5, cM, and 4.4 cM, respectively. The published purple stigma locus, which may be allelic to the Ps-4 (t) gene from Asian cultivated rice, is tentatively named Ps-4 (t).