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目的建立用于测定兔血清中淫羊藿苷和朝藿定A、B、C含量的方法,并进行血药浓度监测。方法采用C18固相微萃取功能化移液枪头处理兔血清,超高效液相色谱法测定血清中4种成分的含量。色谱条件:Capcell C_(18)色谱柱(250 mm×4.6 mm,5μm),乙腈-3%醋酸(24∶76)为流动相,流速为1m L·min-1,检测波长为270 nm。结果 4种淫羊藿黄酮苷与血清中的其他成分能很好分离。朝藿定A、B、C和淫羊藿苷均在0.05~10.00 mg·L-1内呈现良好线性关系,平均回收率分别为98.82%、97.12%、97.43%和96.59%。灌胃给药后8 h内,可测得血清中淫羊藿苷和朝霍定A、B、C等成分的含量。结论建立的方法灵敏度高,准确,操作简便,可为淫羊藿的药动学研究提供检测方法。
OBJECTIVE To establish a method for the determination of Icariin and Epimedin A, B and C in rabbit serum and to monitor the blood concentration. Methods The rabbit serum was processed by C18 solid phase microextraction and functional liquid pipette tip. The contents of four components in serum were determined by ultra performance liquid chromatography. The chromatographic conditions were as follows: Capcell C 18 column (250 mm × 4.6 mm, 5 μm), acetonitrile-3% acetic acid (24:76) as mobile phase at a flow rate of 1 mL · min-1 with a detection wavelength of 270 nm. Results 4 kinds of Epimedium flavonoid glycosides and other components of the serum can be well separated. Epimedin A, B, C and icariin showed a good linear relationship in the range of 0.05-10.00 mg · L-1, with the average recoveries of 98.82%, 97.12%, 97.43% and 96.59%, respectively. 8 h after intragastric administration, measurable serum icariin and Chaohuo Ding A, B, C and other ingredients. Conclusion The established method is sensitive, accurate and easy to operate. It can provide a method for the pharmacokinetic study of Epimedium.