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目的:观察慢性应激肝郁大鼠肾上腺髓质中NE合成酶TH及其转录因子c-fos的神经元数目、平均光密度、基因表达变化及逍遥散的干预作用。方法:将大鼠随机分为对照组、模型组、逍遥散治疗组,束缚应激造模后第22天取出肾上腺髓质,用免疫组化和RT-PCR检测c-fos、TH的表达。结果:模型组大鼠肾上腺髓质中c-fos、TH的阳性神经元数目、平均光密度值,均大于对照组(P<0.05),基因表达均上调(P<0.01)。给予逍遥散治疗后,c-fos、TH阳性神经元数目、平均光密度值减少,基因表达下调(P<0.01)。结论:慢性应激中,大鼠肾上腺髓质去甲肾上腺素能神经元被激活,TH及其c-fos表达增加,而逍遥散可以有效的进行干预。
Objective: To observe the neuron number, average optical density and gene expression of NE synthase TH and its transcription factor c-fos in adrenal medulla of chronic stress-induced liver depression in rats and the intervention effect of Xiaoyaosan. Methods: The rats were randomly divided into control group, model group and Xiaoyaosan treated group. Adrenal medulla was removed on the 22nd day after restraint stress. The expressions of c-fos and TH were detected by immunohistochemistry and RT-PCR. Results: The number of c-fos and TH positive neurons and the average optical density in the adrenal medulla of model group were significantly higher than those of the control group (P <0.05), and the gene expression was up-regulated (P <0.01). After Xiaoyaosan treatment, the number of c-fos, TH-positive neurons decreased, mean optical density decreased and gene expression decreased (P <0.01). CONCLUSION: In chronic stress, the adrenal medulla noradrenergic neurons are activated, the TH and c-fos expression are increased, and Xiaoyaosan can effectively intervene.