论文部分内容阅读
目的探讨检测 K-ras 基因突变在胰腺癌诊断中的作用。方法收集15例胰腺癌,33例非胰腺癌病人的外周血标本,提取血浆 DNA。采用限制性酶切片段长度多态性-多聚酶链反应(RFLP-PCR)方法检测突变的 K-ras 基因,直接测序方法测出 K-ras 基因的突变类型。结果胰腺癌病人外周血浆 K-ras 基因突变率为73%(11/15),非胰腺癌组中有2例胰头肿块型慢性胰腺炎者发生K-ras 基因突变,其余均为阴性;血浆 K-ras 基因突变与肿瘤部位、大小及分期无关。结论检测外周血浆 K-ras 基因突变可用于胰腺癌的辅助诊断和胰腺癌高危人群的筛选。
Objective To explore the role of detecting K-ras gene mutation in the diagnosis of pancreatic cancer. Methods The peripheral blood samples of 15 cases of pancreatic cancer and 33 cases of non-pancreatic cancer were collected to extract plasma DNA. The mutated K-ras gene was detected by restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) and the K-ras gene mutation type was determined by direct sequencing. Results The K-ras gene mutation rate in peripheral blood of patients with pancreatic cancer was 73% (11/15). K-ras gene mutation was found in 2 cases of pancreatic head mass type chronic pancreatitis in the non-pancreatic cancer group. The rest were negative; K-ras mutations have nothing to do with tumor location, size and stage. Conclusion Detection of peripheral K-ras mutations in plasma can be used for the diagnosis of pancreatic cancer and screening for high-risk pancreatic cancer.