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为了更全面地研究双斑东方鲀三倍体诱导方法,促进双斑东方鲀产业健康发展,采用静水压法,探讨了诱导双斑东方鲀三倍体的起始时间、压强大小和处理时间长度等的最适诱导条件。实验结果表明:在水温18~20℃时,授精后5 min,采用10~15 Mpa的压强、5~7 min的处理时间的静水压诱导条件,可获得96.67%以上的三倍体诱导率,孵化率亦能达77%以上。本文首次建立了双斑东方鲀的三倍体苗种静水压诱导法,获得了高效的双斑东方鲀三倍体诱导流程,并为其他东方鲀类的生产性三倍体诱导研究提供了参考。
In order to study the three-body induction method and promote the healthy development of the two-spotted eastern family, the hydrostatic pressure method was used to study the initiation, pressure and processing time Length of the optimal conditions for the induction. The experimental results showed that the induction rate of the triploid reached 96.67% at the water temperature of 18 ~ 20 ℃, 5 min after insemination, under the pressure of 10 ~ 15 Mpa and the hydrostatic pressure of 5 ~ 7 min. , Hatching rate can reach more than 77%. In this paper, the hydrostatic induction method of triploid seedlings of two-spotted Oriental catfish was established for the first time, and the efficient three-body induction process of two-spotted Oriental catfish was obtained and provided the basis for the study of productive triploidy induction in other orientations. reference.